How are members of Enterobacteriaceae distinguished from each other?

How are members of Enterobacteriaceae distinguished from each other?

How can members of the Enterobacteriaceae and Pasteurellaceae be distinguished from each other? Members of Enterobacteriaceae are oxidase negative, while those of Pasteurellaceae are oxidase positive. Members of the Enterobactericeae produce siderophores.

What biochemical test is used to distinguish between the Enterobacteriaceae and the Pasteurellaceae?

Pasteurellaceae are typically rod-shaped, and are a notable group of facultative anaerobes. Their biochemical characteristics can be distinguished from the related Enterobacteriaceae by the presence of oxidase, and from most other similar bacteria by the absence of flagella.

What is Enterobacteriaceae testing?

Enterobacteriaceae is used mainly as an indicator organism for the potential presence of Salmonella and other bacteria in the EB family. Testing within the industry has shifted from primarily using coliform as an indicator organism to using Enterobacteriaceae as an indicator.

Which of the following is diagnostic for Neisseria meningitidis?

Neisseria are small, gram-negative cocci readily identified with Gram stain and by other standard bacteriologic identification methods. Serologic methods, such as latex agglutination and coagglutination tests, allow rapid presumptive diagnosis of N. meningitidis in blood, cerebrospinal fluid, synovial fluid, and urine.

Which of the following is utilized to distinguish between members of the family Enterobacteriaceae and differentiate them from other Gram-negative rods?

virtually all Enterobacteriaceae perform a one-step reduction of nitrate to nitrite. The nitrate test differentiates them from Gram-negative rods that either do not reduce nitrate or reduce it beyond nitrite to N2 or other compounds.

Why cant Enterobacteriaceae be distinguished by a Gram stain?

A gram stain distinguishes between gram positive and gram negative bacteria. However, the Enterobacteriaceae is a family of all gram negative bacteria. The gram stain would not be helpful because all Enterobacteriaceae would give the same result.

How are the family Enterobacteriaceae described?

Enterobacteriaceae is a family of Gram-negative, facultatively anaerobic, non-spore-forming rods. Characteristics of this family include being motile, catalase positive, and oxidase negative; reduction of nitrate to nitrite; and acid production from glucose fermentation. However, there are also many exceptions.

Which group of tests best identifies the Morganella and Proteus general?

Cards

Term Blood culture collection , clean with	Definition 2% iodine and then 70% alcohol
Term Which group of test best identifies Morganella and proteus?	Definition Motility, urease and PAD.

Why is it important to identify Enterobacteriaceae?

Some members of the Enterobacteriaceae have had a large impact on infectious diseases, clinical microbiology and public health. They are involved in diarrhoeal diseases and recognised as one of the major bacterial food-borne pathogens.

How do you test for Enterobacteriaceae?

Tests for identification of members of Enterobacteriaceae family

1. Citrate utilization Test.
2. Indole Test.
3. Motility Test.
4. Methyl Red (MR) Test.
5. VogesProskauer (VP) Test.
6. Triple Sugar Iron (TSI) Agar Test.
7. Urease Test.

Which is member of the Enterobacteriaceae cause food spoilage?

This group includes a full array of microorganisms, including all coliform bacteria. Its members run the gamut from bacteria like Salmonella and E.coli well-known to cause foodborne illness to food spoilage agents to various microorganisms normally present in the human intestinal tract as part of the gut flora.

How many species of Enterobacteriaceae are there?

Enterobacteriaceae is a large family of Gram-negative bacteria. It was first proposed by Rahn in 1936, and now includes over 30 genera and more than 100 species.

How do you differentiate Neisseria gonorrhoeae and Neisseria meningitidis?

N. gonorrhoea is kidney shaped with apposing ends concave. N. meningitidis is semicircular diplococcus with flat apposing ends.

Is Neisseria meningitidis encapsulated?

Neisseria meningitidis meningitidis organisms are encapsulated, or surrounded by a polysaccharide capsule. This capsular polysaccharide is used to classify N. meningitidis into 12 serogroups. Six of these serogroups cause the great majority of infections in people: A, B, C, W135, X, and Y (12).

Which of the following carbohydrates does Neisseria meningitidis produce acids from?

Neisseria meningitidis Cells are cocci occurring in pairs and they utilize glucose and maltose to produce acid.

Which of the following differential tests are use to differentiate members of the family Enterobacteriaceae?

Sulfur Indole Motility Media (SIM) Sulfur Indole Motility Media (SIM) This is a differential medium. It tests the ability of an organism to do several things: reduce sulfur, produce indole and swim through the agar (be motile). SIM is commonly used to differentiate members of Enterobacteriaceae.

What are the different tests used to differentiate Staphylococcus spp from Streptococcus spp?

Staphylococcus and Micrococcus spp. are catalase positive, whereas Streptococcus and Enterococcus spp. are catalase negative. If a Gram-positive cocci is catalase positive and presumed to be a staphylococci, the coagulase test is often performed.

Why is it important to differentiate glucose Nonfermenters from Enterobacteriaceae?

Why is it important to differentiate glucose nonfermenters from Enterobacteriaceae? -because nonfermenters are more highly resistant to common antimicrobial agents. Why is pure culture important when identifiying bacteria?

Are all members of the family Enterobacteriaceae capable of glucose fermentation?

All members of the Enterobacteriaceae family ferment glucose with acid production and reduce nitrates. Certain physiological groups of organisms may be recognized within Enterobacteriaceae. Psychrotrophic members of this family are not uncommon, although the Enterobacteriaceae are regarded widely as being mesophilic.

What diagnostic test differentiates Proteus and Providencia species from other Enterobacteriaceae?

The diagnostic test that differentiates Proteus and Providencia species from other Enterobacteriaceae is the urease test. The urease test identifies organisms that can hydrolyze urea to produce carbon dioxide and ammonia.

How do Pseudomonas and Enterobacteriaceae differ?

aeruginosa is an aerobic bacterium that is known as a common nosocomial pathogen. The key difference between P. aeruginosa and Enterobacteriaceae is that P. aeruginosa is a bacterial species while Enterobacteriaceae is a family of gram-negative bacteria.

What general characteristics do members of the family Enterobacteriaceae have in common?

Members of the family Enterobacteriaceae have the following characteristics: They are gram-negative rods, either motile with peritrichous flagella or nonmotile; grow on peptone or meat extract media without the addition of sodium chloride or other supplements; grow well on MacConkey agar; grow aerobically and …

How many sub categories of Enterobacteriaceae are there?

Currently, there are 51 genera within the family Enterobacteriaceae, which are Arsenophonus, Biostraticola, Brenneria, Buchnera, Budvicia, Buttiauxella, Cedecea, Citrobacter, Cosenzaea, Cronobacter, Dickeya, Edwardsiella, Enterobacter, Erwinia, Escherichia, Ewingella, Gibbsiella, Hafnia, Klebsiella, Kluyvera, Leclercia …

What is the primary differentiation of Enterobacteriaceae bacteria based on?

A procedure is described for identifying members of the family Enterobacteriaceae isolated from clinical specimens. The methods are based on primary differentiation of the various groups of bacteria by the use of Kligler Iron Agar and lysine-iron-agar.

How do you differentiate Proteus mirabilis and Proteus vulgaris?

Organisms that swarm on 5% sheep blood agar, exhibit a characteristics odor, and are oxidase negative can be presumptively identified as Proteus spp. With further testing by spot indole, the positive isolates may be presumptively reported as Proteus vulgaris and the negative ones as Proteus mirabilis.

Which group of tests best identifies the Morganella and Proteus genera quizlet?

When a solution of ferric chloride is added, the iron reacts with phenylpyruvic acid, forming a green-colored complex. Phenylalanine deaminase is found in the genera Morganella, Providencia, and Proteus and is an excellent test to determine if an organism belongs to this group.

How do you differentiate between a Proteus species and Salmonella species?

-Salmonella is urease negative. Proteus is urease positive, making this medium useful in differentiating Proteus from other Gram-negative rods.

Where can you find Enterobacteriaceae?

Enterobacter are ubiquitous in nature; their presence in the intestinal tracts of animals results in their wide distribution in soil, water, and sewage. They are also found in plants.

What can all Enterobacteriaceae do?

Enterobacteriaceae can cause a wide range of illnesses, which include wound infections, urinary tract infections, gastroenteritis, meningitis, pneumonia, septicemia, and hemolytic uremic syndrome. Not all are regarded as truly pathogenic some are regarded as opportunistic.

How can you differentiate between Pseudomonas aeruginosa and Salmonella?

Pseudomonas colonies are fluorescent under this spectrum of light. you can use oxidase test, pseudomonase =+ve and salmonella = -ve. also you can use urea media positive for pseudomonas and negative for salmonella.