Is Bacillus subtilis catalase positive?

It was renamed in 1872 by Ferdinand Cohn. Bacillus subtilis (B. subtilis) is a Gram-positive, aerobic bacterium. It is rod-shaped and catalase-positive.

Is Bacillus pumilus catalase positive or negative?

Biochemical Test and Identification of Bacillus subtilis

Basic Characteristics Properties (Bacillus subtilis)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Flagellated
Gas Negative (-ve)

Is Bacillus subtilis nitrate positive?

The Gram-positive soil bacterium Bacillus subtilis, generally regarded as an aerobe, grows under strict anaerobic conditions using nitrate as an electron acceptor and should be designated as a facultative anaerobe.

Is Bacillus subtilis positive for oxidase test?

The gram-positive endospore-forming bacterium Bacillus subtilis has, under aerobic conditions, a branched respiratory system comprising one quinol oxidase branch and one cytochrome oxidase branch. The system terminates in one of four alternative terminal oxidases.

Is Bacillus subtilis Gram-positive or Gram negative?

Bacillus subtilis is the best-characterized member of the Gram-positive bacteria.

Is Bacillus subtilis Voges Proskauer positive?

It was found that the bacteria are Gram positive spore chain Bacillus.

Is Bacillus subtilis acid fast positive or negative?

Bacillus Subtilis, Blue for Non-Acid Fast.

What type of hemolysis is Bacillus subtilis?

Bacillus subtilis is a facultative anaerobic Gram-positive non-pathogenic bacterium that includes members displaying hemolytic activity. To identify the genes responsible for hemolysis, a random mariner-based transposon insertion mutant library of B. subtilis 168 was constructed.

Is Bacillus megaterium Gram-positive?

B. megaterium is a Gram-positive, aerobic spore-forming neutralophilic bacterium found in diverse habitats but commonly regarded as a soil bacterium.

Are Bacillus species oxidase positive?

All of the B. alvei strains examined were highly oxidase positive.

Does Bacillus subtilis ferment glucose?

subtilis cannot ferment either glucose or pyruvate efficiently (unlike E. coli) and why pyruvate enhances glucose fermentation are unknown. NMR analysis showed that fermentation products in B. subtilis include acetate, acetoin, ethanol, lactate, succinate and 2,3-butanediol, indicating a mixed acid fermentation [10].

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Is Bacillus anthracis aerobic or anaerobic?

Bacillus anthracis is an aerobic spore-forming bacterium that causes disease in humans and animals. The bacteria is found in two forms: cutaneous anthrax and inhalation anthrax.

Is Staphylococcus aureus urease positive or negative?

Biochemical Test and Identification of Staphylococcus aureus

Basic Characteristics Properties (Staphylococcus aureus)
Urease Positive (+ve)
VP (Voges Proskauer) Positive (+ve)
Fermentation of
Arabinose Negative (-ve)

What is catalase test?

This test is used to identify organisms that produce the enzyme, catalase. This enzyme detoxifies hydrogen peroxide by breaking it down into water and oxygen gas. The bubbles resulting from production of oxygen gas clearly indicate a catalase positive result.

Is Bacillus megaterium oxidase positive or negative?

Penicillin-Susceptible, Oxidase-Negative, Nonhemolytic, Nonmotile Bacillus megaterium in Disguise of Bacillus anthracis.

Why Bacillus subtilis is Gram-positive?

Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants and humans. …

Bacillus subtilis
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae

What phylum is Bacillus subtilis?

bacterial phylum Firmicutes Bacillus subtilis, a low %G+C, Gram-positive, endospore-forming member of the bacterial phylum Firmicutes, is found predominately in the soil and in association with plants.

Is Bacillus subtilis endospore formed?

Bacillus subtilis is a spore forming, motile, rod-shaped, Gram-positive, facultative aerobe. … subtilis is used as a model organism for studying endospore formation in bacteria. Endospores of B. subtilis can tolerate harsh environmental conditions, such as UV exposure and high temperatures.

How do you become a VP test?

Procedure of Voges–Proskauer (VP) Test

  1. Prior to inoculation, allow medium to equilibrate to room temperature.
  2. Using organisms taken from an 18-24 hour pure culture, lightly inoculate the medium.
  3. Incubate aerobically at 37 degrees C. …
  4. Following 24 hours of incubation, aliquot 2 ml of the broth to a clean test tube.
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Is Bacillus cereus urease positive?

It was positive for urease, catalase, oxidase, deoxidization of nitrate, and Voges−Proskauer reaction. However, it was negative for methyl red, indole, H 2 S production, and phenylalanine deaminase reaction tests.

How do you know if an endospore is positive or negative?

positive/negative: endospores stain green and may be seen inside cells or outside cells. endospore negative cells stain red with no evidence of green staining. ex. bacillus subtilis and clostridium are examples of endospore positive bacteria.

What does a positive Endospore stain look like?

The presence of endospores in a bacterial culture can be detected by staining with malachite green. Because the endospore coat is so tough, steam is used to enable dye penetration. After washing, only the endospores will retain the primary stain Malachite green.

What color is endospore negative?

Principle of Dorner’s method for staining endospores Since the counterstain nigrosin is negatively charged, bacterial cells don’t easily take up the counterstain. Therefore, vegetative cells appear colorless, endospores stain red, and the background is black.

Is Bacillus subtilis alpha hemolytic?

subtilis and B. pumilus were small and greenish, suggesting alpha-hemolysis (results not shown). The isolated strains, which were weakly positive, were further analyzed for the hemolytic activities of their cell-free supernatants.

Is Staphylococcus aureus hemolytic?

Staphylococcus aureus is a common pathogen causing both hospital and community-acquired infections. Hemolysin is one of the important virulence factors for S. aureus and causes the typical β-hemolytic phenotype which is called complete hemolytic phenotype as well.

Why is Bacillus subtilis a model organism?

Bacillus subtilis is the best studied model organism of the Gram-positive lineage. It is naturally transformable and has an extremely powerful genetic toolbox. … It is an important industrial organism, being proficient at secreting proteins and making small fine chemicals, as well as acting as a plant growth promoter.

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Is Staphylococcus epidermidis Gram-positive or negative?

Staphylococcus epidermidis which is known as a coagulase-negative and Gram-positive Staphylococcus, is one of the five significant microorganisms that are located on human skin and mucosal surfaces with the ability of causing nosocomial infections due to the wide usage of medical implants and devices, hence until 1980 …

What is the gram reaction of Staphylococcus aureus?

Staphylococci are gram-positive aerobic organisms. Staphylococcus aureus is the most pathogenic; it typically causes skin infections and sometimes pneumonia, endocarditis, and osteomyelitis. It commonly leads to abscess formation.

What is the morphology of Bacillus subtilis?

Bacillus subtilis morphology describes rod-shaped, Gram-positive bacteria that show up on both positive and negative Gram stain techniques. A bacterial rod is a symmetrical cylinder with rounded ends. A significant difference in pressure across the cytoplasmic membrane pushes the cell wall into a specific shape.