What are Akita mice?

A spontaneous genetic rodent model that closely recapitulates aspects of human diabetes is the Akita mouse, which carries an autosomal dominant allele consisting of a missense mutation in the Ins2 gene.

What is DB db mice?

db/db Mice (JAX™ Mice Strain) Details db/db mice are used to model phase 1 to 3 of diabetes type II and obesity. Mice that are homozygous for the diabetes spontaneous mutation (Lepr^db) demonstrate morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and come to be hypoinsulinemic.

What is the ob gene?

The ob gene, an adipose specific gene believed to encode a satiety factor that regulated food intake and energy expenditure, has recently been discovered in mice. Two separate mutations in the mouse ob gene result in the development of morbid obesity.

Can mice get diabetes?

The endocrine system of mice is similar to that of humans, so mice can develop both type 1 and type 2 diabetes. Autoimmune destruction of the pancreatic beta cells leading to lack of insulin production is the main characteristic of type 1 diabetes.

When do NOD mice develop diabetes?

Based on data from the NOD/ShiLtJ strain, NOD mice maintained under SPF conditions develop insulitis between 2 and 4 weeks in females and within 5 to 7 weeks in males. Diabetes develops in 90% to 100% of females by 30 weeks; in contrast, only 50% to 80% of males.

What is leptin made of?

Leptin (from the Greek word leptos, meaning “thin”) is derived from the lep gene, located on chromosome 7, which transcribes a 167 amino acid peptide with a molecular weight of 16kD.

Is the ob gene found in humans?

Comparative mapping of mouse and human DNA indicated that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. We mapped the human OB gene on a yeast artificial chromosome (YAC) contig from chromosome 7q31.

Where is the leptin receptor located?

hypothalamus The leptin receptor protein is found on the surface of cells in many organs and tissues of the body, including a part of the brain called the hypothalamus.

How do you detect diabetes in mice?

Mice were considered to have diabetes if blood glucose levels were above 250 mg/dL for two sequential blood glucose tests. Mice were considered to have prediabetes when mouse blood glucose levels were in the range between 150 and 250 mg/dL for two sequential blood glucose tests.

How do you collect mouse urine?

On the lid, grasp mouse from behind and seize the skin around neck and scapular region using your thumb and index finger of your left hand and hold the mouse. Mouse may urinate because of stress by holding or abdominal pressure. Collect urine using a pipette if urine remains on a tray or a wire mesh.

How do diabetics get mice?

To induce diabetes, animals of both sexes received daily intraperitoneal (i.p.) injection of STZ for 5 consecutive days at 55 mg/kg BW (a dose that is known to induce diabetes in male mice) or for females, 75 mg/kg BW of STZ.

Why are SCID mice used?

What are SCID mouse models typically used for? SCID models are typically used in biology studies of the immune system. SCID mouse models are also used for tumor growth studies and studies of normal and abnormal lymphocyte development and function.

What are non obese diabetic mice?

Non-obese diabetic or NOD mice, like biobreeding rats, are used as an animal model for type 1 diabetes. Diabetes develops in NOD mice as a result of insulitis, a leukocytic infiltrate of the pancreatic islets. The onset of diabetes is associated with a moderate glycosuria and a non-fasting hyperglycemia.

What does nod stand for in mice?

NOD, also known more fully as NOD/ShiLtJ or NOD/LtJ, is an inbred strain of mouse that serves as a very popular model for diabetes research (“NOD” stands for “Non-Obese Diabetic”).