What is bDNA used for?

Molecular diagnostic assays using bDNA technology for detection of nucleic acid target molecules are sensitive, specif- ic, and reliable tools in the diagnosis of viral and bacterial infections and for monitoring disease progression during the course of therapy.

How does bDNA work?

The bDNA assay uses a 96-well microplate format, and is based on a series of specific hybridization reactions and chemiluminescent detection of hybridized probes. Attached to the surface of each microwell are capture probes that contain a specific nucleotide sequence.

What does bDNA technopedia mean?

This product can leverage Business DNA (BDNA) Technopedia, which is a structured catalog of hardware and software product releases that provides clean, consistent baseline information about products and manufacturers.

What is branched chain DNA?

BDNA assay (Branched-chain DNA assay) is a powerful multi-application molecular diagnostic technique based on signal amplification of available nucleic acid in a given sample. … FDA approved the bDNA technology for HIV-1 and HCV viral load testing, so as to monitor disease progression in HIV-1 and HCV patients.

What does bDNA stand for?

bDNA

Acronym Definition
bDNA Branched DNA (biochemistry)
bDNA Bangladesh Diploma Nurses Association
bDNA Brentwood-Darlington Neighborhood Association (Portland, OR)
bDNA Beclomethasone Dipropionate Nasal Aerosol (medication)

How does transcription mediated amplification work?

What are types of DNA?

There are two types of DNA in the cell autosomal DNA and mitochondrial DNA. Autosomal DNA (also called nuclear DNA) is packaged into 22 paired chromosomes. In each pair of autosomes, one was inherited from the mother and one was inherited from the father.

What is a DNA and B DNA?

A-DNA is thought to be one of three biologically active double helical structures along with B-DNA and Z-DNA. It is a right-handed double helix fairly similar to the more common B-DNA form, but with a shorter, more compact helical structure whose base pairs are not perpendicular to the helix-axis as in B-DNA.

Is situ a hybridization?

In situ hybridization is a laboratory technique in which a single-stranded DNA or RNA sequence called a probe is allowed to form complementary base pairs with DNA or RNA present in a tissue or chromosome sample. The probe has a chemical or radioactive label attached to it so that its binding can be observed.

What is a capture probe?

A branched DNA assay begins with a dish or some other solid support (e.g., a plastic dipstick). The dish is peppered with small, single stranded DNA molecules (or chains) that ‘stick up’ into the air. These are known as capture probe DNA molecules.

Is RNA branched?

RNA molecules with 2,5-branched linkages (Figure 1) are well-recognized intermediates in RNA splicing by both group II introns and the spliceosomes of higher organisms (13).

Is DNA branched or unbranched?

Both DNA and RNA are long, unbranched polymers of nucleotides. Each nucleotide consists of a heterocyclic base linked via a five-carbon sugar (deoxyribose or ribose) to a phosphate group (see Figure 4-1).

What is amplified in the ligase chain reaction?

The ligase chain reaction (LCR) is a method of DNA amplification. … Thus, LCR requires two completely different enzymes to operate properly: ligase, to join probe molecules together, and a thermostable polymerase (e.g., Taq polymerase) to amplify those molecules involved in successful ligation.

What is the TMA method?

TMA involves the isothermal amplification of rRNA by reverse transcription and subsequent generation of numerous transcripts by RNA polymerase. Following amplification, these RNA copies are hybridized with a complementary oligonucleotide probe for detection via a chemiluminescent tag (Figure 2).

Is DNA helicase involved in PCR?

In living organisms, a DNA helicase is used to separate two complementary DNA strands during DNA replication (Kornberg & Baker, 1992). … As the DNA helicase unwinds dsDNA enzymatically, the initial heat denaturation and subsequent thermocycling steps required by PCR can all be omitted.

Is TMA quantitative?

TMA/HPA offers several advantages over conventional qualitative and quantitative RT-PCR techniques. It is rapid, with a total assay time of less than 4 h, and requires only small amounts of RNA, less than 500 ng per triplicate analysis.

What are the 3 main functions of DNA?

DNA now has three distinct functionsgenetics, immunological, and structuralthat are widely disparate and variously dependent on the sugar phosphate backbone and the bases.

What are 5 facts about DNA?

Here are 6 weird but true facts about DNA.

Where is your DNA located?

cell nucleus Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA). Mitochondria are structures within cells that convert the energy from food into a form that cells can use.

Why is B-DNA right-handed?

As in B-DNA, the two complementary strands in A-DNA are antiparallel and form right-handed helices. Normal DNA undergoes transition from the B to A form under drying. In A-DNA, the base pairs are planar but their planes form a considerable angle with the axis of the double helix.

What are the 3 types of DNA?

Three major forms of DNA are double stranded and connected by interactions between complementary base pairs. These are terms A-form, B-form,and Z-form DNA.

Why is Z-DNA left-handed?

Z-DNA is a left-handed helical form of DNA in which the double helix winds to the left in a zigzag pattern. DNA containing alternating purine and pyrimidine repeat tracts have the potential to adopt this non-B structure in vivo under physiological conditions, particularly in actively transcribed regions of the genome.

What is in situ analysis?

In biology and biomedical engineering, in situ means to examine the phenomenon exactly in place where it occurs (i.e., without moving it to some special medium). … For example, examining a cell within a whole organ intact and under perfusion may be in situ investigation.

What is hybridization in biology?

Hybridization is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing.

What is the principle of hybridization?

The principle of hybridization analysis is that a single-stranded DNA or RNA molecule of defined sequence (the probe) can base-pair to a second DNA or RNA molecule that contains a complementary sequence (the target), with the stability of the hybrid depending on the extent of base pairing that occurs.