With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.
What is bodipy used for?
The combination of these desirable attributes makes BODIPY fluorophores attractive as tools in a variety of applications, for example, in biochemical labeling, light-emitting devices, supramolecular fluorescent gels, light harvesting systems, and as sensitizers in solar cells.
What does nile red stain do?
Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells.
Can bodipy be fixed?
We routinely add BODIPY 493/503 from a 1 mg/ml stock to fixed or living cells at a final concentration of 1 μg–10 μg/ml; followed by at least a 1-h incubation at 37°C. The final concentration of BODIPY 493/503 depends on fat droplet size, the larger the fat droplets, the less stain is needed.
How do you stain with bodipy?
Procedure
- Grow cells under culture conditions relevant for the study. …
- At the time-point of interest, prepare 2 μM BODIPY staining solution in PBS. …
- Wash cells with a quick rinse using 3 ml PBS to remove media/serum.
- Incubate on BODIPY staining solution in the dark for 15 min at 37 °C.
Is Nile red fluorescent?
Nile red exhibits properties of a near-ideal lysochrome. It is strongly fluorescent, but only in the presence of a hydrophobic environment. The dye is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
What color is BODIPY?
Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.
Is BODIPY water soluble?
BODIPY dye (A) is highly water-soluble. … BODIPY dye A displays weak fluorescence in 0.5 M phosphate buffer solution (PBS) (pH 7.40) with fluorescence quantum yield of only 4.2% while it becomes highly fluorescent with fluorescence quantum yields of 68% and 61% in methylene chloride and ethanol, respectively.
Is BODIPY cell permeable?
To test our hypothesis, we chose boron-dipyrromethene (BODIPY) as the model fluorescent dye in our study. … However, BODIPY indeed suffer problems of low-water solubility and poor membrane permeability 27 , 28.
Is Nile red toxic?
TOXICITY AND IRRITATION NILE RED: No significant acute toxicological data identified in literature search. All waste must be handled in accordance with local, state and federal regulations.
What ethnicity is Nile red?
He is of German descent.
Is Nile Red Dead?
NileRed on Twitter: I’m not dead. I just severely underestimated how long it would take to move and build a new lab
How do you detect lipid droplets?
Lipid droplets can be visualized either by staining the lipid ester core using fluorescent dyes or by labeling lipid droplet-specific proteins using antibodies.
How does filipin bind to cholesterol?
Filipin is a naturally fluorescent polyene antibiotic that binds to cholesterol but not to esterified sterols. … Filipin fluorescence is observed with UV excitation around 360 nm and emission around 480 nm. Filipin binding perturbs the bilayer structure, so filipin cannot be used on living cells.
Where are lipid droplets found?
adipose tissue Lipid droplets, also referred to as lipid bodies, oil bodies or adiposomes, are lipid-rich cellular organelles that regulate the storage and hydrolysis of neutral lipids and are found largely in the adipose tissue.
Which stains could be used to label lipids?
The Oil Red O (ORO) stain can identify neutral lipids and fatty acids in smears and tissues. Fresh smears or cryostat sections of tissue are necessary because fixatives containing alcohols, or routine tissue processing with clearing, will remove lipids. The ORO is a rapid and simple stain.
How do you make red Nile dye?
The protocol is simple and rapid: Nile red is diluted from a stock solution (0.4 mg/ml in DMSO) 200-fold into water to 2 μg/ml, and a 10-fold volume excess (e.g., 50 ml staining solution for a 5 ml minigel) is added to a gel with immediate and thorough agitation.
Is Nile red water soluble?
What is Nile Red, Product 19123, soluble in? Nile Red is soluble in methanol (1 mg/mL), ethanol (1mg/mL), water (0.2 mg/mL), and 2-methoxyethanol (1mg/mL).
Is Nile red cell Permeant?
Cell-permeant TAXOL®-based probes for imaging the microtubule cytoskeleton in live cells. Available with blue, green, or far-red fluorescence.
Who is Nigel Braun?
Nigel Braun began posting his chemistry experiments to YouTube in 2014 under the name NileRed (after the lipophilic stain) with no intention of amassing such a large audience. But now, four years later, he has over 420,000 subscribers that tune in regularly to watch his latest experiment.
What’s Nile red?
Nile red is a fluorescent organic dye used in various imaging studies in biology. The colour that it fluoresces is dependent on what solvent it is dissolved in. For example, in water it fluoresces strongly blue, whereas in toluene (a solvent commonly found in paint strippers) it fluoresces yellow.
Is BODIPY polar?
BODIPYs show good stability both to environmental changes (such as pH [6]) and light [7]. Yet, due to the nonpolar nature of BODIPY core, they tend to aggregate in highly polar solvents [8].
Is BODIPY aromatic?
3. BODIPYs fused to aromatic heterocycles. So far, instead of aromatic hydrocarbons, many aromatic ring-fused BODIPY dyes have been developed by introducing 5-membered aromatic heterocycles, such as furan, thiophene and pyrrole derivatives, at the b bond, as summarized in Fig.
Is boron a fluorescent?
The boron fluorescence quantum yields are below to 1%, this is due to intramolecular rotation [16]. Thus, the dependence of the fluorescence quantum yield of ligand 1 and compound 2 on the viscous medium was studied using methanol and glycerol binary mixture.
Does DMSO quench fluorescence?
The presence of dimethyl sulfoxide (DMSO) even in concentrations as low as 1% decreases the yield of fluorescence. Neglecting this quenching leads to the erroneous conclusion that DMSO in a concentration dependent manner inhibits the thapsigargin induced histamine release from peritoneal rat mast cells.
Graduated from ENSAT (national agronomic school of Toulouse) in plant sciences in 2018, I pursued a CIFRE doctorate under contract with Sun’Agri and INRAE in Avignon between 2019 and 2022. My thesis aimed to study dynamic agrivoltaic systems, in my case in arboriculture. I love to write and share science related Stuff Here on my Website. I am currently continuing at Sun’Agri as an R&D engineer.