What is brain homogenate?

(hō-moj’ĕ-nāt), Tissue ground into a creamy consistency in which the cell structure is disintegrated (so-called cell-free).

How do I make my brain homogenate?

Procedure:

  1. Weigh frozen brain. …
  2. Homogenise approx. …
  3. Transfer homogenate to 2L beaker on ice/salt. …
  4. Repeat homogenisation : 4 x 20g brain to create a pool of 1L of 10%-brain homogenate.
  5. Dispense 100 x 0.5 mL into pre-labelled, Nalgene 1.2mL cryovials, immerse in bleach (10%-Chloros) for >15 minutes at 4oC.

How do you make liver tissue homogenate?

What is the right method to get liver homogenate?

  1. put liver in a glass homogenizer and add nidefold volume of 0.25M sucrose solution(with 1mM EDTA)(10% W/V)
  2. homogenize liver and move 2ml of homogenate into 2ml eppendorf tube.
  3. do centrifuging at 700x g for 10 min to separate debris and nuclear.

How do you homogenize your liver?

Homogenization of Liver Tissue

  1. Cut liver into appropriately sized pieces for analysis (10mg-300mg).
  2. OPTIONAL: Wash tissue 3x with ~1mL PBS. …
  3. Samples 100mg or greater. …
  4. Add 0.025mL to 0.6mL buffer (2 volumes of buffer for every volume of sample).
  5. Close the microcentrifuge tubes.
  6. Place tubes into the Bullet Blender®.

What is the pH of liver homogenate?

Peptide SCX chromatography separation at pH 2.65 of a liver homogenate fraction.

What is a liver homogenate?

For example, a liver homogenate is a liver tissue in which its cells have been mechanically disrupted, releasing the organelles and cytoplasm. The word homogenate was introduced by VR Potter in1941 (J.