Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits. How do you prepare Janus green B solution?
To prepare a Janus Green B solution, use a 1% aqueous solution. Add 1 g of Janus Green B to 75 ml of DI water. Dilute to a final volume of 100 ml with DI water.

Which stain is used to observe mitochondria?

Janus green stain To observe mitochondria in the live cells, Janus green stain is used. Which stain is used for staining of living cell?
Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable.

What do you mean by vital staining?

Vital stain means a stain that can be applied on a living cells without killing them. Thus these stains have been useful for both diagnostic and surgical techniques in a variety of medical specialities. How does Janus green work?

Janus Green B is a basic dye and vital stain used in histology. … The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.

Frequently Asked Questions(FAQ)

Why is Janus green used to stain mitochondria?

(d) Janus green is used to stain mitochondria. Janus green act as an indicator and changes colour according to the amount of oxygen present. It oxidizes to blue colour in. presence of oxygen and in its absence changes its colour to pink.

What is the colour of Janus green B solution?

Specifications

Appearance Blue color solution
Absorption maxima, λmax1 (50% ethanol) 651 – 677 nm
Absorption maxima λmax2 (50% ethanol) 397 – 403 nm

What is Green B?

B sustainable, today. On the occasion of Earth Day, Benetton Group presents GREEN B, the project that rounds up all the brand’s initiatives for sustainability. “GREEN B is the ambassador of Benetton’s innate green soul, the symbol of everything that is sustainable in us.” …

What is the importance of Diazine green?

by Biology experts to help you in doubts & scoring excellent marks in Class 10 exams. Diazene Green solution is added to the Glucose solution in anaerobic respiration experiment to check the presence of oxygen in glucose solution.

How can we prevent the entry of gases into glucose solution?

Which cell organelle is known as suicidal bag?

Lysosomes Lysosomes are called suicide sacks. They are produced by the Golgi body. They consist of a single membrane surrounding powerful digestive enzymes. It acts as the “garbage disposal” of the cell by breaking down cell components that are no longer needed as well as molecules or even bacteria that are ingested by the cell.

Which stain is used while observing onion cells?

According to the question, the stain used in onion root cells is acetocarmine as it stains the nucleus and helps in viewing different stages of mitosis.

Which stain is used for mounting of onion cells?

Which stain is used for mounting of onion cells ? by Biology experts to help you in doubts & scoring excellent marks in Class 10 exams. Acetocarmine. … Question : Which stain is used for mounting of onion cells ?

Question Which stain is used for mounting of onion cells ?
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Why is iodine used to stain onion cells?

The layers of an onion contain simple sugars (carbohydrates) some of which are stored as starch (starch granules). … Although onions may not have as much starch as potato and other plants, the stain (iodine) allows for the little starch molecules to be visible under the microscope.

Why it is called Supravital stain?

The term vital stain is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive and unfixed, outside the body, supravital stains are temporary in nature.

What are the disadvantages of staining cells?

The disadvantages of histology and histological staining include: Preparation of the slides using the paraffin technique can be time-consuming; frozen slides are faster to prepare, but this can affect the resolution, especially when using light microscopy.

What is progressive staining?

Progressive staining occurs when the hematoxylin is added to the tissue without being followed by a differentiator to remove excess dye. Modified progressive staining occurs when a mild differentiator is used not to remove excess stain from the nuclei, but rather removes background staining.

What stains connective tissue?

Mason’s Trichrome stain is used to highlight connective tissue fibers in a tissue section. Collagen fibers are stained a blue hue, nuclei a blue-black, and cytoplasm, keratin, and muscle fibers a range of pink to red hues. AZAN Trichrome. AZAN Trichrome stain is used to highlight collagen fibers within a tissue section …

What is the importance Flouroscent staining?

A fluorescent staining procedure has been developed which rapidly, accurately, and economically measures the viability of mycobacterial cells. … These cells appear green when viewed under incident ultraviolet illumination. Ethidium bromide enters dead cells and intercalates between the bases of DNA molecules.

Why basic dyes are often used in microbiology?

Basic Dyes carry a positive charge & are more used for staining than Acidic dyes. This is because opposite charges attract, basic dyes stain the negatively charged components of cells including nucleic acid & many proteins.

Why would you use two different dyes when staining cells?

By using different stains, one can preferentially stain certain cell components, such as a nucleus or a cell wall, or the entire cell.

Which of the following dye is best suited for staining chromosome?

Caramine is best suited for staining chromosomes. Carmine is the bright red dye that is mainly used for color food, textiles and cosmetics.

What is the use of mitochondria?

Mitochondria are membrane-bound cell organelles (mitochondrion, singular) that generate most of the chemical energy needed to power the cell’s biochemical reactions. Chemical energy produced by the mitochondria is stored in a small molecule called adenosine triphosphate (ATP).

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