What is NB4 cell line?

What is NB4 cell line?

NB4 is an acute promyelocytic leukemia cell line that has been shown to be inducible to terminal neutrophil maturation with all-trans retinoic acid (ATRA). HL60 cells are differentially inducible with 12-O-tetradecanoylphorbol-13-acetate (TPA) or dimethyl sulfoxide (DMSO) to monocytes or granulocytes, respectively.

What is NB4?

NB4, a maturation inducible cell line with t(15;17) marker isolated from a human acute promyelocytic leukemia (M3). … The induction of apoptosis and cell cycle arrest by arsenic trioxide in lymphoid neoplasms.

What is cell line study?

Cell lines have revolutionized scientific research and are being used in vaccine production, testing drug metabolism and cytotoxicity, antibody production, study of gene function, generation of artificial tissues (e.g., artificial skin) and synthesis of biological compounds e.g., therapeutic proteins.

What is APL leukemia?

Acute promyelocytic leukemia (APL) is an aggressive type of acute myeloid leukemia (AML). It happens when there are too many of the blood-forming cells called promyelocytes in the blood and bone marrow.

What causes APL?

APL is caused by a chromosomal translocation (rearrangement of material) that occurs in some of the body’s cells during a person’s lifetime (a somatic mutation ). The translocation involves the fusion of two genes : the PML gene on chromosome 15 and the RARA gene on chromosome 17.

How do I choose a cell line?

The Do’s and Dont’s of Choosing a Cell Line

  1. Don’t Choose Simply Because It Is Used in the Literature. …
  2. Do Consider If It Fits Your Biological Model. …
  3. Do Consider the Experiments You Want to Perform. …
  4. Table 1: Features of commonly used cell lines. …
  5. Don’t Assume That All Cell Lines Have the Same Culturing Requirements.

What are the types of cell line?

Understanding Types of Cell Lines

  • Frog cell lines.
  • Hamster cell lines.
  • Mouse cell lines.
  • Rat cell lines.
  • Dog cell lines.
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What is cell line authentication?

We offer a human cell line authentication (CLA) service that uses Promega’s GenePrint 10 system to confirm the identity of human cell lines through short tandem repeat (STR) analysis and to detect the presence of cell line contaminants.

Can APL be cured?

Acute promyelocytic leukemia (APL) has become a curable disease by all-trans retinoic acid (ATRA)-based induction therapy followed by two or three courses of consolidation chemotherapy. Currently around 90% of newly diagnosed patients with APL achieve complete remission (CR) and over 70% of patients are curable.

Can APL be cured as of 2021?

ATRA and ATO have greatly modified the prognosis of APL. APL is now considered a curable disease.

How treatable is APL leukemia?

Because of advances in diagnostic techniques and modern treatments, APL is today considered to be the most curable subtype of acute myeloid leukemia in adults, with complete remission rates of 90 percent and cure rates of approximately 80 percent and even higher among low-risk patients.

What diagnosis is APL?

Acute promyelocytic leukemia (APL) is a blood cancer characterized by a marked increase in a type of white blood cells known as promyelocytes, a type of immature white blood cell. It develops in about 600 to 800 individuals each year in the United States, most often in adults around the age of 40.

How do I confirm my APL diagnosis?

The diagnosis of APL is based on an evaluation of the clinical presentation, morphology, immunophenotyping, karyotype, RT-PCR, FISH and immunofluorescence with anti-PML monoclonal antibodies. The redundancy of diagnostic tests is beneficial because of the broad and indistinct characterization of the disease.

Which type of leukemia is most fatal?

Patients with the most lethal form of acute myeloid leukemia (AML) based on genetic profiles of their cancers typically survive for only four to six months after diagnosis, even with aggressive chemotherapy.

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What is a normal cell line?

An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro.

What is A549 cell line?

A549 (ATCC CCL-185) is a human lung adenocarcinoma cell line initiated through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. The cells are adherent with an epithelial morphology.

Which is an example of human breast cell line?

We characterized 84 cell lines based on the status of three important receptors conventionally used for breast cancer subtyping, i. e. , estrogen receptor (ER), progesterone receptor (PR), and human epithelial receptor 2 (HER2), and classified them using the same nomenclature, i. e. , luminal A, luminal B, HER2 …

Why do we use CHO cells?

CHO cells became popular because they have a low chromosome number and were used to study genetics. … These characteristics have made CHO cells the most important cell line for the production of therapeutic proteins including humanized antibodies.

What is secondary cell line?

Secondary cell culture refers to cell lines that have been immortalized, usually by overexpressing an enzyme called human telomerase reverse transcriptase (hTERT), and can divide indefinitely. Many tissue types are not amenable to immortalisation, so primary cell culture may be the only option in some circumstances.

Where are mammalian cells found?

Mammalian cells are cells that are derived or isolated from tissue of a mammal. In this experiment, students are introduced to four mammalian cell types: fibroblasts, epithelial cells, lymphocytes and macrophages. Lymphocytes are found within the blood.

How are cell lines validated?

Phenotypic methods, such as cell morphology and growth curve analysis, are perhaps the simplest methods used to validate cell lines.

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Why is cell line characterization important?

Why is cell line characterization important? Cell line characterization is critical to the control of biological product manufacturing. The objective of the testing is to confirm the identity, purity, and suitability of the cell substrate for manufacturing use.

What is an STR profile?

STR profiling is an analytical DNA technique which PCR-amplifies variable microsatellite regions from a genomic DNA template, separates the PCR amplicons on a genetic analyzer, and uses software to analyze the resulting data and compare the data from one specimen to databases housing previously generated STR sets.

Which leukemia is treated with vitamin A?

ATRA is a form of vitamin A that is typically part of the initial (induction) treatment of APL. It is given either along with chemo, or along with arsenic trioxide for the initial treatment of APL.

How long does APL treatment take?

The most common options for maintenance therapy are ATRA alone, or ATRA along with chemo (6-mercaptopurine (6-MP) and/or methotrexate). Maintenance therapy is typically given for about a year.

Is APL genetic?

APL is not inherited . The condition arises from a translocation in some of the body’s cells ( somatic cells ) that occurs after conception. This is referred to as a somatic mutation . Somatic mutations may affect the individual by causing cancers or other diseases, but they are not passed on to offspring.