What is phenol red used for cell culture?

Phenol red is used as a pH indicator. The sodium salt of Phenol Red is widely used in culture media to identify changes of neutral to acidic pH values. A Phenol Red solution has: A yellow color at a pH of 6.4 or below.

What does phenol red indicate?

3.5 Acid formation from carbohydrates and organic acids The phenol red is the pH indicator and is initially neutral (pH 7). When the bacterium is inoculated into the tube, the bacterium that ferments the sugar will result in the production of acid that will change the color of phenol red to yellow.

What is the purpose of phenol red in viral cell culture?

Phenol red is a known pH indicator widely used in cell culture for detecting the pH change of the culture medium during the whole culture process.

Why is phenol red added to culture media?

Phenol red is widely used in cell culture medium as a pH indicator, because of its color sensitivity at pH range of 6.8 to 8.2. Within this pH range, its color exhibits a gradual transition from yellow to red.

What does phenol red do in media?

Phenol red (PR) is the standard pH indicator in various cell and tissue culture media, as it provides a quick check for the health of the culture. PR has also been used in multiple protocols to detect cellular hydrogen peroxide as well as peroxidase activity from human peroxidase enzymes.

What happens when phenol red turns yellow?

Phenol red is a pH indicator that is yellow at a pH below 6.8 and red at a pH above 7.4 with varying shades from yellow to red between those pH levels. If the indicator has turned yellow in the bottle this means it has become contaminated with something that has made the pH more acidic and brought the pH below 6.8.

How does phenol red indicate pH?

Phenol red exists as a red crystal that is stable in air. … A solution of phenol red is used as a pH indicator, often in cell culture. Its color exhibits a gradual transition from yellow (λ_max = 443 nm) to red (λ_max = 570 nm) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink (fuchsia) color.

Is phenol red toxic?

First of all, if ingested phenol red is poisonous and can cause vomiting and diarrhea. If the compound gets into your eyes, it can also be dangerous, so avoid touching your eyes after handling phenol red.

Is phenol red a carcinogen?

OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Inhalation – May cause respiratory irritation. Inhalation May be harmful if inhaled.

What does the phenol red test test for?

What is this? Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria. It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose).

How do you use phenol red?

What elements are in phenol red?

Chemical Structure Description The carbon atoms in the chemical structure of PHENOL RED are implied to be located at the corner(s) and hydrogen atoms attached to carbon atoms are not indicated – each carbon atom is considered to be associated with enough hydrogen atoms to provide the carbon atom with four bonds.

Why cell culture media is red?

Most of the commercially available culture media include phenol red as a pH indicator, which allows constant monitoring of pH [18]. During the cell growth, the medium changes color as pH is changed due to the metabolites released by the cells. … Medium is bright red for pH 7.4, the optimum pH value for cell culture.

Is phenol red a pure substance or mixture?

Note: Because phenol red is a solution of the dye compound dissolved in water, it is actually composed of two pure substances.

Is phenol red light sensitive?

Note that phenol red is a chromophore that will absorb light and thus decrease the effective illumination of a sample. More importantly, phenol red can contribute to background and can even mark lysosomes in cells illuminated with near UV light.

What is the purpose of phenol red in the fermentation tube?

What is the purpose of the phenol red in the carbohydrate fermentation broth? The phenol red is the pH indicator in the experiment. As fermentation occurs, acid may be a byproduct. More acid produced would reduce the pH of the solution.

Is phenol red a reagent?

Phenol Red, Reagent, ACS is mostly used as a pH indicator that is commonly known due to it being used in pool water testing kits.

What is the pH of phenol red?

6.2 to 8.2 Phenol red, also known as phenolsulfonphthalein, is a pH indicator dye that exhibits a gradual transition from yellow to red over a pH range of 6.2 to 8.2 (Figure 2). Above 8.2 the dye turns a bright fuchsia color.

What does methyl red test for?

The methyl red (MR) test detects the production of sufficient acid during the fermentation of glucose and the maintenance of conditions such that the pH of an old culture is sustained below a value of about 4.5, as shown by a change in the colour of the methyl red indicator which is added at the end of the period of …

Why is phenol red orange?

A color change from red- orange to yellow occurs when acid is produced as a result of carbohydrate metabolism. This medium may be used for many species of bacteria due to the high growth-promoting qualities of casein peptone. Carbohydrates may be added to the broth base in a final concentration of 1%.

Is phenol red soluble?

Additionally, Phenol Red could potentially be useful as a differentiation factor. Solubility : Soluble in water yielding a dark red solution (25 mg/ml), alcohol (4 mg/ml), methanol, ethanol (200 mg/ml), and aqueous alkali hydroxides or carbonates with formation of a red colored solution.

How do you make phenol red indicator?

Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). After the solution is effected, add sufficient water to produce 100 ml.

What color is the pH indicator phenol red at acidic pH?

yellow pH indicator; neutral (red) to acidic (yellow) values.

Can you drink phenol red?

Nausea, vomiting, abdominal pain, and diarrhea are common symptoms after exposure to phenol by any route. Ingestion of phenol can also cause severe corrosive injury to the mouth, throat, esophagus, and stomach, with bleeding, perforation, scarring, or stricture formation as potential sequelae.

Can phenol red go down the drain?

Do not discard any phenol, chloroform, or TRIzol™ down the sink drain.

How do you store phenol red?

Wash hands thoroughly after handling. Storage: Store in General Storage Area [Green Storage] with other items with no specific storage hazards. Store in a cool, dry, well-ventilated, locked store room away from incompatible materials. Use ventilation to keep airborne concentrations below exposure limits.

Is phenol red the same as phenolphthalein?

Is phenolphthalein the same as phenol red? … Answer 2: Phenolpthalein is very similar to phenol red. In fact, most of the molecule is identical, except for a small group that dangles off the side of the main structure.

Is phenol red solution flammable?

Fire hazard : Flammable liquid and vapour. Explosion hazard : Heating may cause a fire or explosion. Protection during firefighting : Do not attempt to take action without suitable protective equipment.

What happens when you mix baking soda calcium chloride and phenol red?

Description: This video shows a chemical reaction of sodium bicarbonate (baking soda), calcium chloride (road salt), and an indicator phenol red. The chemicals react to form calcium carbonate, sodium chloride, and carbon dioxide gas. This changes the pH inside the bag resulting in a color change in the phenol red.